CRUSTACEA FROM THE BOLSÓN OF CUATRO CIÉNEGAS, COAHUILA, MEXICO
GERALD A. COLE
Route 4, Box 892
Flagstaff, Arizona 86001
ABSTRACT
Twelve crustaceans are known from the Cuatro Ciénegas basin. These include: the cirolanid isopods Speocirolanathermydronis, Sphaerolana interstitialis, Sphaerolana affinis; one stenasellid isopod Mexistenasellus Coahuila,the first of the Stenasellidae found in the New World; and two endemic hadzioid (weckeliid) amphipods originallyassigned to Mexiweckelia, M. colei, and M. particeps. M. particeps will be referred to the monotypic new genusParamexiweckelia (Holsinger, in press). These six species were described from the basin and are endemic to it; thegenera Sphaerolana and Paramexiweckelia have no representatives outside the basin. Other crustaceans include: aspecies of the brine shrimp, Artemia; another anostracan, probably a species of Streptocephalus; an harpacticoidcopepod similar to Cletocamptus albuquerquensis; an ostracod referable to Chlamydotheca; an amphipod much likeHyalella azteca, although designated here simply Hyalella; and the decapod Palaemonetes suttkusi. The lastspecies, Chlamydotheca, and H. azteca were described originally from Mexican material. Although crustaceandiversity is low in its thermal waters, discovery of more species can be expected in the Cuatro Ciénegas basin.
RESUMEN
Doce crustáceos son conocidos de la cuenca de Cuatro Ciénegas . Estos incluyen: los isópodos cirolánidosSpeocirolana thermydronis, Sphaerolana interstitialis, Sphaerolana affinis; un isópodo estenasellidoMexistenasellus Coahuila, el primer Stenasellidae descubierto en el Nuevo Mundo; y dos anfípodos hadziidos(weckeliid) endémicos originalmente asignados a Mexiweckelia, M. colei y M. particeps. Se referirá a M. particepscomo un nuevo género monotípico, Paramexiweckelia (Holsinger, en prensa). Dichas seis especies fueron descritasde la cuenca donde son endémicas; los géneros Sphaerolana y Paramexiweckelia no tienen representantes afuera dela cuenca. Otros crustáceos son: una especie de brine shrimp, Artemia; un anóstraco probablemente una especie deStreptocephalus; un copépodo harpacticoide parecido a Cletocamptus albuquerquensis; un ostrácodo referable aChlamydotheca; un anfípodo parecido a Hyalella azteca, y designado aqui Hyalella; y el decápodo Palaemonetessuttkusi. Chlamydotheca y las dos ultimas especies fueron descritas originalmente de muestras mexicanas. Aunque ladiversidad de crustáceos sea baja en las aguas termales de la cuenca de Cuatro Ciénegas , se espera el descubrimiento demas especies locales.
INTRODUCTION.-The history of crustacean studies in Cuatro Ciénegas is largely a saga of serendipity, and lucky"firsts" in what soon became a series of advances in Mexican zoology. Many people contributed their energy andpersonal talents to the field work, W. L. Minckley being the most avid collector of us all. From the beginning, Dr.Thomas E. Bowman, Division of Crustacea at the Smithsonian Institution, sustained and guided us. His counsel wasinvaluable, and on two occasions he was the perfect host while I was in Washington examining specimens and literaturehoused in the U. S. National Museum. Furthermore, he was kind enough to read this manuscript and to criticize itconstructively. Other people to whom I am indebted for pertinent information are Drs. Denton Belk, John R. Holsingerand Glenn Longley.
It is the purpose of this paper to tell how and where various new species were found and to detail what happenedthereafter. The paper will deal with: three new species of cirolanid isopods and one new genus; one new genus andspecies of the stenasellid isopods; and two new species of hadziid amphipods belonging to two new genera. All thespecies and two genera are endemic to the Cuatro Ciénegas basin, and four genera were described originally from CuatroCiénegas material. The status of Mexican carcinology in relation to Cuatro Ciénegas will be discussed, and someprophesies about future discoveries will be ventured. Throughout, there may be a more anecdotal style than is usual inmodem scientific writing!
CRUSTACEAN DIVERSITY IN CUATRO CIÉNEGAS. The impression, perhaps fostered by those who have studiedthe organisms of Cuatro Ciénegas, is one of exciting diversity. This does not apply to the Crustacea, although there isanother side to the coin that will be discussed subsequently. The striking endemism and abundance of new taxa, and theoccurrence of several cirolanid isopods, a group that is poorly represented in non-marine settings, may have contributedto the notion of high diversity.
Actually, the number of crustaceans from the valley of Cuatro Ciénegas is not impressive; only 12 species have beennoted. The Amphipoda and Isopoda of the Peracarida are fairly abundant, but other major groups are poorly represented.To date, no mysidacean peracaridans have been found, although at least four troglobitic species are known from México(Bowman 1982c).
Among the Branchiopoda, a population of the brineshrimp genus Artemia Leach 1819, was sampled 20 March 1973 inLaguna Salada by Denton Belk. Also, some immature anostracans have been collected at various times from ephemeralroadside pools; they are probably some species of Streptocephalus Baird 1852. No representatives of the Cladocera,however, have been reported from the basin. The Copepoda are represented by an harpacticoid close to, if notconspecific with, Cletocamptus albuquerquensis (Herrick) 1895.1/ Among the other "entomostracans", some speciesof the ostracod Chlamydotheca Saussure 1858 is abundant, serving as a food item for the endemic box turtle,Terrapene coahuila Schmidt and Owens 1944. At least five species of Chlamydotheca have been noted from Méxicoto date.
Amphipods referable to Hyalella Smith 1874, designated H. azteca (Saussure) 1858 in many papers concerned withCuatro Ciénegas fauna, are ubiquitous. Palaemonetes suttkusi Smalley 1964, a decapod glass or grass shrimp, occursin some lagunas in the basin. Chlamydotheca, Hyalella azteca and Palaemonetes suttkusi were described initiallyfrom Mexican specimens (Saussure 1858; Smalley 1964). The endemic crustaceans that were described later amount tosix species. Thus 75% of the Cuatro Ciénegas Crustacea have Mexican affinities, and 50% are endemic.
The reason for the low crustacean diversity in the thermal and hypogean waters of Cuatro Ciénegas may be rooted in theextreme stability of such habitats. Recently, Ward and Stanford (1983) discussed animal diversity in lotic environments.In two instructive plots (their Fig. 1 , p. 349, and Fig. 2, p. 351) the authors showed highest diversity in communitiessubjected to intermediate levels of disturbing incidents. Lowest diversities occur in thermal springs where constancyprevails, and at the opposite end of the spectrum in extremely disturbed habitats such as those subjected to frequent acidmine discharges or to heavy organic loading.
The thermal, interstitial, and subterranean waters of Cuatro Ciénegas are stable habitats that may account for(in additionto low diversity) the presence of cirolanid and stenasellid isopods as well as hadziid amphipods. Flabelliferan isopods(including some parasitic families as well as the free-living Cirolanidae and Sphaeromatidae), the Stenasellidae, andamphipods of the family Hadziidae have not been successful in colonizing the common types of epigean freshwaterenvironments. The same can be said for the mysidacean Peracarida, abundant in the seas, but largely troglobitic orhalophilic when inland. Mysis relicta Lovén 1861, occurring in the deeps of northern oligotrophic lakes, may be anexception to this. The asellid isopods and gammarid amphipods, by contrast, have invaded lakes, springs, and streamsfar from the sea and have, in some instances, moved directly from epigean freshwaters into caves.
Bowman (1981) concluded that flabelliferan isopods have been excluded from what we consider normal epigeanfreshwater habitats by the competition of aquatic insects, especially the predaceous species, and their naiads, nymphs,and larvae. Insects occur but rarely in caves and hot springs, the inland habitats where the non-parasitic flabelliferans arefound. In the Cuatro Ciénegas basin one small, cold-water laguna contains many benthic mayfly naiads, but noflabelliferan isopods, nor stenasellid isopods, nor hadziid amphipods as far as is known.
Ward and Stanford (1982) discussed the importance of temperature in the ecologic evolution of insects, pointing out thatthe original home of aquatic insects was in the cool headwaters of streams. From there they moved downstream andspread to warmer waters. Very few species, however, invaded and prospered in thermal waters. Shuster (1981)remarked on the absence of predaceous insects in a hot spring in Socorro, New México, where a population offlabelliferan isopods (Sphaeromatidae in this instance) thrives. He attributed this, however, to intense predation by theomnivorous isopod, Thermosphaeroma thermophilum (Richardson) 1897.
After stating that the diversity of crustaceans in the Cuatro Ciénegas environments is low, it is time to point out that itmight be much higher than we suspect. First, the difficulty in collecting some of the species leads to the conclusion thatthere are more microhabitats and new species awaiting discovery. The work of Dickson et al. (1979) implies thathypogean crustaceans have normal levels of genetic variability, comparable to that of epigean forms. Isolatingmechanisms, then, could lead to speciation in the various interstitial, subterranean, and thermal habitats of the CuatroCiénegas basin. Second, as Frey (1982) emphasized, the notion of cosmopolitanism in the cladoceran Crustacea blindedworkers to the host of undescribed species around us in North and South America. This probably applies to thecyclopoid copepods and ostracods also. Diligent searching at shallow margins of lagunas, pozos, and in aquatic weedbeds might uncover the presence of some Cladocera, Copepoda, and Ostracoda even though they have not been taken inplankton collections from the bolsón. Careful scrutiny, involving modem techniques, or even old-fashioned methodsbased on morphology, could well reveal many new and perhaps endemic species.
Another point to consider hinges on the abundance of weckeliid amphipods in various waters of Cuatro Ciénegas(Holsinger and Minckley 1971). Recently, Stock (1982) presented statistical evidence that hadziid amphipods andcyclopoids are mutually exclusive in West Indies habitats. Validating this generality on the basis of the weckeliidmembers of the Hadziidae would make a worthwhile research project in the basin. The important factor in the relationshipseems to be the predatory nature of the amphipods. Moreover, Stock found the thermosbaenacean crustaceans andhadziids rarely co-occur, yet the former and cyclopoids are associated in a normal fashion in West Indies groundwaters.At this time only one thermosbaenacean species is known from the mainland of North America; this is Monodellatexana Maguire 1965, from the Edwards Plateau, Texas. Suitable hypogean habitats that lack weckeliid amphipodsmight be the best places to find cyclopoid copepods and, possibly, crustaceans closely related to Monodella in theCuatro Ciénegas valley.
Several accounts of the various habitats in the Cuatro Ciénegas basin have noted the ubiquity of Hyalella azteca(Minckley 1969; Cole and Minckley 1970, 1972; Holsinger and Minckley 1971). In the years since those reports at leasttwo new species of Hyalella have been described in the American Southwest. These are H. texana Stevenson andPeden (1973) and H. montezuma Cole and Watkins (1977). The idea of one species, H. azteca, ranging from SouthAmerica to the treeline in North America is on the wane, and it is not accurate to assign a specific name to the Coahuilahyalellids as has been done. The color variation among different populations in the waters of Cuatro Ciénegas is striking,and one unique population, especially, comes to mind: one spring contains snow-white individuals with black eyes.Probably there are many species masquerading as Hyalella azteca and some of these may be in the bolsón of CuatroCiénegas.
The concept of cosmopolitanism has oversimplified the taxonomy of Artemia. Therefore, it is unrealistic to designateanimals from North America as A. salina (Linnaeus) 1758, described originally from Lymington, Southhampton,England. Clark and Bowen (1976) recommended that the term A. salina be dropped for the brine shrimps (found on sixcontinents), except for, perhaps, the type locality. Their paper marked the starting point for a more realistic examinationof brine shrimp populations, and presented evidence for far more heterogeneity than had been suspected previously. TheArtemia from Laguna Salada has yet to acquire a specific name.
Other species of the Anostraca can be anticipated in the Cuatro Ciénegas basin. Streptocephalus linden Moore 1966,S. machine Moore 1966, Thamnocephalus mexicanus Linder 1941, and T. platyurus Packard 1879 have beenreported from Coahuila, although not from Cuatro Ciénegas (see Belk 1975). Five other species of Streptocephalushave been reported from Texas, or Mexican states bordering Coahuila. There are, therefore, many possibilities as towhat the ultimate taxonomic designation will be when applied to the immature forms from Cuatro Ciénegas.
It is not illogical to assume that more faunal surprises await us as more subterranean habitats are investigated in theCuatro Ciénegas bolsón. The African shrimp genus, Potamalpheops, is represented in North America by a singleMexican species, the troglobitic, white, eyeless P. stygicola (Hobbs 1983). This relict decapod recalls the formerconnection of the present African and American continents and leads to predicting that careful searching or serendipitousgood fortune will add to the unique faunal list of Cuatro Ciénegas .
ISOPODA, FLABELLIFERA.-Cirolanidae. The first crustacean described as a new species from the Cuatro Ciénegasbasin was the cirolanid isopod, Speocirolana thermydronis Cole and Minckley (1966). At that time the genusSpeocirolana was represented by two other species, both Mexican. Bolívar (1950) reported an isopod from a San LuisPotosí cave, assigning it to the marine genus Cirolana Leach 1818, but placing it in a new subgenus, Speocirolana,and giving it the specific name pelazei. Three years later, Rioja (1953) described a new form, Cirolana(Speocirolana) bolivari, collected in a Tamaulipas cave. Bowman (1964) raised Bolívar's subgeneric nameSpeocirolana to full generic rank. Thus, S. thermydronis became the third certain species of the genus. It is probablethat Conilera stygia, described by Packard (1900) on the basis of animals collected in springs near Monterey, NuevoLeón, is another species of Speocirolana, although it has not been found since Packard's report and, apparently, notypes were deposited (T. E. Bowman, pers. comm.). Certainly it is not a member of the marine genus Conilera Leach1818, to which it was assigned by Packard.
The animals referable to Speocirolana are much like typical marine cirolanids except for their eyeless condition and lackof pigmentation. They have five obvious pleonites (abdominal segments) anterior to the pleotelson, although the lateralmargins of the fourth and fifth are reduced, and the genus represents the third of nine types of pleonal segmentation inorder of increased fusion of pleonites (Bowman 1975a, Fig. 4). Its category shows it slightly more modified thanCirolana and Conilera, both of which show only a slight reduction of the fifth pleonite; they belong to Bowman'ssecond type. The exopod and endopod are well developed on each uropod, forming a typical crustacean biramousappendage. A major character of the genus is that the first three pairs of pereopods are prehensile, followed byambulatory appendages 4-7. Packard's Conilera stygia also has subchelate, prehensile pereopods 1-3, if we are to relyon his words of 1900, "Only the first three pairs of legs are short, with a very thick hand; the four hinder pairs are longand slender."
With the published description of Speocirolana thermydronis, only four genera of cirolanid isopods were known fromthe mainland of North and Middle America. These included: Cirolanides texensis Benedict 1896 from caves of theEdwards Plateau of Texas; three species of Speocirolana, (perhaps four if Conilera stygia can be assigned to thatgenus); Creaseriella anops (Creaser) 1936 from the Yucatán Peninsula; and Antrolana lira Bowman 1964 from aVirginian cave. All are troglobitic forms. Nine years later another blind, unpigmented cirolanid, Mexilana saluposiBowman (1975a), was named from a San Luis Potosí cave, raising the total genera to five.
In 1982 Contreras-Balderas and Purata-Velarde published an account of another species, Speocirolana guerrai. Thedescription of the new species was based on a study of 48 specimens from a small cave near Linares, Nuevo León. In thesame journal, in the paper directly following the description of S. guerrai, the fifth and sixth species of Speocirolanawere named S. pubens and S. endeca from San Luis Potosí and Tamaulipas, respectively (Bowman 1982a)..Speocirolana endeca earned its name by being the llth species of troglobitic Mexican cirolanid to be described (seeBowman 1982a, p. 23).
Bowman's two species of Speocirolana nicely fit the distributional pattern formed by the first four species andConilera stygia (Contreras-Balderas and Purata-Velarde 1982, Figs. 28-32). The geographic alignment, from S.pelazei and S. pubens in San Luis Potosí to the most northern species, S. thermydronis, closely follows the UpperJurassic coastline according to Burckhardt (1930) and Imlay (1943). With reference to the boundaries of marineembayments in México proposed by Axelrod (1979), the Speocirolana species are found near the old Paleocene andEocene shorelines. A pre-Tertiary origin for the genus seems reasonable. The isopods probably moved directly fromretreating seas into interstitial and hypogean habitats without an intermediate epigean freshwater stage. The location ofPackard's "Conilera " within the north-south line formed by the Speocirolana species prompted Contreras-Balderasand Purata-Velarde (1982, p. 10) to write " . . . se reafirma la suposcion de que 'Conilera' stygia Packard debiorepresentar una Speocirolana
Speocirolana thermydronis was described on the basis of one 15-mm female collected in April, 1964, by Mary L.Allesio, a member of a University of Colorado Museum field group. The specimen was sent to us by Clarence J.McCoy. Twenty-nine more animals were collected in August, 1967, and additional distributional and descriptive dataresulted from that (Minckley and Cole 1968a). We were apprehensive about describing a new species from a study ofonly one specimen, but the earlier descriptions of S. pelazei and S. bolivari were so detailed that we were certain thatthe Cuatro Ciénegas isopod was different. We were not the first in the annals of hypogean carcinology to describe a newtaxon from such scanty material; two more instances will be mentioned in later pages.
Many European and North American zoologists have invaded México to investigate the nation's unique crustacean fauna.It is, therefore, worthy of mention and indeed a happy thought that 50% of the species of Speocirolana (if we excludeConilera stygia) were described by Mexican scientists!
The discovery of the new genus Sphaerolana Cole and Minckley (1970) was owed to William S. Brown. Brown, thena graduate student at Arizona State University, was studying the box turtle Terrapene coahuila. Stomach contents ofsome preserved Terrapene specimens in Tempe contained remains of isopods, which Brown asked me to examine. Theisopods were in very poor condition, but obviously were unique. I though they might belong to the flabelliferan familySphaeromatidae, because many were rolled tightly in a sphere. Brown detailed the location of the pozo from which theturtles had been collected (immediately christened Pozo Tortuga by Cole and Minckley) and the next summer, August1967, we began searching for isopods in the same pool. A few were found when travertine blocks were broken apart,but the results were generally disappointing. Later we discovered the best way to collect the white, eyeless isopods wasto pull up emergent vegetation at pool margins and examine the muddy roots; the crustaceans were abundant there. Soonwe had adequate study material and we sent specimens to Dr. Bowman at the Smithsonian Institution. He observed thatthe mouthparts were typical of a cirolanid isopod rather than a sphaeromatid, and that the isopod could not be referred toany known genus. The two family names were combined to create the new generic name for the isopod, SphaerolanaCole and Minckley 1970. Since then no isopods referable to Sphaerolana have been discovered outside the CuatroCiénegas basin.
Although two other blind, white cirolanids are capable of rolling into a sphere, they are fundamentally different fromSphaerolana. These are Creaseriella Rioja 1953 from the Yucatán Peninsula and Faucheria Dollfus and Viré 1905from caves in France. Creaseriella resembles Speocirolana in having five well-developed pleonites anterior to thepleotelson, the last two having indistinct lateral margins in both genera. Sphaerolana has but two abdominal segmentscephalad to its pleotelson, and the caudal one has indistinct, covered lateral margins. Faucheria reveals four pleoniteswith well-developed lateral margins, but the transverse sutures are incomplete, representing nearly fused segments. It isthe last type in Bowman's (1975a, Fig. 4) nine categories arranged in order of increasing fusion of pleonites.Sphaerolana characterizes the eighth category.
The uropodal rami of Creaseriella are strong, with large endopods and exopods. Those of Sphaerolana are scarcelyrepresented and are inserted in a lateral notch near the end of the protopod. In Faucheria the uropodites are representedby subterminal, reduced structures on the protopod. The uropods of Sphaerolana have evolved further from the typicalcirolanid type. All the pereopods of the Yucatán Creaseriella are ambulatory; the first three pairs are subchelate andprehensile in Faucheria and Sphaerolana, as they are in members of Speocirolana. There seems to be no closerelationship among the three sphere-forming genera. Probably the ability to roll has developed independently in thecirolanids.
Our early specimens of Sphaerolana (S. interstitialis) came from habitats clustered near the northern tip of the
Sierra de San Marcos (see Minckley 1969, Fig. 2; Cole and Minckley 1970, Fig. 40; Holsinger and Minckley 1971, Fig.4). The most remote site was 6 km south of the tip near the eastern slope. Serendipity played a role in the discovery ofW. affinis in a pozo 12 km southeast of the nearest known S. interstitialis. The pit was carpeted with Chara exceptfor the central, deepest portion, where water entered; there, clearly visible, were clean, white pebbles. The funnel-shapedpit stirred our imaginations to such an extent that we named it Funnel Poso [sic], Pozo Embudo being beyond ourcollective linguistic talent. Dwight W. Taylor, always on the alert for new mollusc habitats, asked George L. Batchelderto dive down carrying a small sieve and to bring back a sample of the pebbles and, perhaps, associated gastropods. Atthat time, August 1967, Batchelder was a graduate student and he complied. To our surprise, the sieve contents includedsome eyeless, white isopods that we immediately recognized as belonging to Sphaerolana. We assumed they wereconspecific with those taken earlier near the tip of Sierra de San Marcos. Later, when it was possible to dissect andexamine them microscopically, several differences emerged, and the small funnel-shaped pozo, about 2 km northeast ofEjido Santa Tecla and about 20 km southeast of the town of Cuatro Ciénegas, became the type-locality for Sphaerolanaaffinis Cole and Minckley 1970. A pattern began to emerge at about that time: the fauna of the eastern part of the basin,and especially the "southeastern lobe", is different from that of the western portion.
Sphaerolana affinis differs from S. interstitialis in details of antenna 2, pleopod 1, pleotelson, and the uropod. Ofthese the most interesting is a small bilobed structure, representing the exopod and endopod of the typical isopod uropod.The two lobes, situated in a shallow pit on the posterolateral border of the protopod, extend slightly beyond the protopodsurface. S. interstitialis is further modified from the typical cirolanid; its uropodites are reduced even more; they arerepresented by only a minute marginal mound within the posterolateral pit. The mound bears two or three setae. Onlythese setae reach and extend past the protopod margin.
At the northern tip of Sierra de San Marcos is a small spring and pool that we christened Taylor's Spring, for reasonsnow forgotten. The small spring is shown as a half-darkened circle in Cole and Minckley (1970, Fig. 40) and as an opencircle in Holsinger and Minckley (1971, Fig. 4). A wide-mouthed quart jar was placed in the spring, its open end tippedupward at an angle of about 350 toward the hole from which the subterranean water entered. Isopods that emergedduring the night were trapped in the jar, although we suspect that many individuals of Speocirolana were able to swimto their freedom. Members of Sphaerolana were unable to crawl up the glassy slope, and most mornings we collectedanimals that had emerged under the cover of darkness. The Sphaerolana individuals were easily separable into two types:one was typically S. interstitialis; the other, on the basis of antenna 2, pleopod 1, pleotelson, and uropod, fitted thedescription of S. affinis. The latter were 1.6-2.4 X the length of any collected in the southeast-lobe habitats, where theyoccurred alone, and were much larger than the co-occurring S. interstitialis in Taylor's Spring. Furthermore, theywere clumsy, elongate, and unable to roll tightly to form a good sphere.
Because the two species were indistinguishable by the unaided eye when sample specimens from allopatric populationswere examined, and readily separable where they coexisted in Taylor's Spring, the notion of character displacement cameto mind immediately. Only eleven years had passed since Brown and Wilson's important paper (1956) entitled "CharacterDisplacement" and meanwhile workers had been confirming the exaggerated differences between sympatric species whencompared with the same characters in allopatric situations. Now, 16 years after the discovery of two kinds ofSphaerolana in Taylor's Spring, an alternative explanation seems reasonable. There could be three species ofSphaerolana in the Cuatro Ciénegas basin. There might be complete reproductive isolation between the typical S.affinis in Funnel Pozo and the elongate, awkward form in Taylor's Spring. Possibly Speocirolana endeca (Bowman1982a) from Tamaulipas should have been S. dodeca!
Sphaeromatidae. At this time no sphaeromatid isopods have been found in Cuatro Ciénegas waters, and so only thecirolanids of the Flabellifera are of concern to us here. It should be mentioned, however, that five species ofThermosphaeroma Cole and Bane 19782/ are found in hot springs of Chihuahua, Texas, and New México. At present,the known species of Thermosphaeroma occur in a north-south line that corresponds closely to the western boundaryof the Maastrichtian (Upper Cretaceous) embayment (see Bowman 1981, Fig. 11) according to Axelrod (1979). Thesesphaeromatid sites are all west of Cuatro Ciénegas. Furthermore, they lie west of all the known species ofSpeocirolana, the Mexican stenasellid isopods, and the New World hadziid amphipods, all forms which have relativesin Cuatro Ciénegas. Some thermal bodies of water in the basin, however, seem to be ideal habitats forThermosphaeroma. Possibly a sphaeromatid population will be discovered there some day.
ISOPODA, ASELLOTA-Stenasellidae. When the original description of Mexistenasellus coahuila was published(Cole and Minckley 1972), we had collected only 10 specimens. The first animals emerged from a small chunk oftravertine that Minckley pulled from the wall of the Laguna Juan Santos. Five specimens in that small travertine blockwere all that were collected in the summer of 1967 despite an intensive search in the laguna and throughout the CuatroCiénegas basin. The following year four more specimens were taken singly from four different habitats, and in 19 70 asingle individual was collected. In each incident the isopod was taken more or less by accident. They were found from 25cm to 3 m below the water surface in soft organic sediment, spongy travertine, and detritus composed of fibrous plantfragments. The waters that yielded these small isopods were definitely warm; their temperatures were all greater than300C, the hottest site being Laguna Escobeda at 34.20.
Although we never learned how or where to collect the stenasellids in Cuatro Ciénegas waters, we know now, thanks toMagniez's (1978) summary of our knowledge of stenasellids, that we were not unique. The French Stenasellus vireiDollfus 1897 was collected in 1896, but despite minutely detailed searching through ensuing years, it was notrediscovered until 1902! Perhaps the stenasellid habit of constructing complex networks of tunnels in compact claysediments, and occupying other intricate interstitial galleries accounts for the difficulty in collecting them. Parenthetically,Adrian Dollfus described Stenasellus virei on the basis of only one individual collected in 1896!
The manuscript describing Mexistenasellus coahuila was submitted first to a journal that was having problems. It wasneglected for about two years before we withdrew it and submitted it to the Proceedings of the Biological Society ofWashington, where it was accepted and published in 1972. Later in the same year two more species ofMexistenasellus were described from San Luis Potosí, M. parzefalli and M. wilkensi (Magniez 1972). Weappreciate Magniez's courtesy in delaying his paper until our publication appeared; he was aware of our find and the longperiod our manuscript had lain without attention. Two years later Argano (1974) published the new M. magniezi fromVeracruz, and later erected the new genus Etlastenasellus to describe E. mixtecus from Oaxaca (Argano 1977).Bowman (1982b) described Mexistenasellus colei from a cave in Tamaulipas, M. nulemex from Nuevo León andEtlastenasellus confinis, the second member of that genus from Oaxaca. Thus, there are eight named stenasellidisopods known from the New World, all from México. Certainly they are more widespread. Since 1976, three specimenshave been collected from deep wells at San Antonio, Texas. They represent an undescribed species (Glenn Longley, inlitt., 11 July 1983). San Antonio is very near the Eocene shoreline shown by Bowman (1981, Fig. 11, from Axelrod1979), where M. nulemex, M. colei, parzefalli and M. wilkensi occur. M. magniezi is farther south on theboundary of the Paleocene marine embayment. The Cuatro Ciénegas location of M. coahuila is west of the Paleoceneboundary, and the two species of Etlastenasellus are south of M. magniezi between the Upper Cretaceous andPaleocene shores.
Guy J. Magniez has continued working on the stenasellids and summarizing our knowledge of them. His studies of thewest African species led him to conclude that the New World Mexistenasellus species are closely related to them,especially Parastenasellus (Magniez 1981). The splitting of Gondwana explains this, implying ancient origins of thestenasellids and suggesting that unknown forms close to Mexistenasellus are awaiting discovery, especially along thenortheastern coast of South America from northern Venezuela to the easternmost tip of Brazil (see Magniez 1981, Fig.1).
In February 1972, when the description of Mexistenasellus was published, about 25 stenasellids were known fromEurope, Asia and Africa. Now, throughout the world, extending as far cast as the island of Borneo, more than 40 speciesin at least eight genera are known. Eight species and two genera occur in México alone, and maybe Glenn Longley'sTexas wells will add another species at least.
Perhaps Mexistenasellus coahuila is the most typical hypogean animal of all that have been found in the thermalwaters of Cuatro Ciénegas. From a study of subterranean animals in the springs of northwestern Yugoslavia and from asurvey of data in the literature, Sket and Velkovrh (1981) concluded that stenasellid isopods are the most characteristicgroup of thermophilous hypogean animals, occupying thermal waters even in tropical regions. Moreover, Magniez(1978) used the stenasellids to answer some general questions concerning the biogeography, ecology, and physiology ofcavernicolous animals found today in temperate regions. At any rate, our find of small red isopods in the travertine blockfrom Laguna Juan Santos may have been the most significant discovery among the Crustacea of Cuatro Ciénegas: thefirst record of the Stenasellidae from the New World.
Asellidae in México. The newly collected specimens of what later proved to be Mexistenasellus coahuila werepuzzling. Although they were obviously asellotes (rather than flabelliferans), we were aware of only one other in theinland waters of México, the so-called Asellus puebla Cole and Minckley (1968) from epigean waters in the states ofPuebla and México. Each Cuatro Ciénegas asellote had two well-developed pleonites anterior to the pleotelson, aprimitive character when compared with the common asellids of the United States and Canada, Lirceus and Asellus;most of the latter are now referable to Caecidotea Packard 1871. The red, eyeless asellotes that emerged from thetravertine in Laguna Juan Santos had never been seen in the New World. We were not prepared for them!
It seems opportune at this time to follow up the story of Asellus puebla because it pertains to Mexican, if not CuatroCiénegas carcinology. Now we believe that Asellus puebla is Caecidotea communes (Say) 1818, described fromstreams around Philadelphia, Pennsylvania, and the first of the inland Asellota to be described in North America. Say'sdescription of his Asellus communes (1818, pp. 427-428) was inadequate in light of modem work, causing muchconfusion for the next century and a half. This began to clear up when Williams (1970) examined specimens collectednear Say's original sites in tributaries of the Schuylkill River near Philadelphia. Williams' excellent redescription revealedthat Cole and Minckley (1968) had busily described a form known for 150 years. How it was introduced to Méxicoremains a problem. It was collected about 101 latitude
farther south than had been reported for any other member of the genus or closely related genera at that time. Since then,however, other asellids referable to Caecidotea have been described from Veracruz (Argano 1972), Chiapas (Bowman1975b), and other southern Mexican and Guatemalan localities (Argano 1977). Suddenly at least five asellids wereknown to occur far south of "Asellus puebla ".
The etymologic history of the North American generic names in the Asellidae was outlined by Bowman (1975b). Briefly,Asellus Geoffroy St. Hillaire 1764, an Old World genus, is different, although at least one Alaskan isopod may belongto it. Caecidotea was coined by Packard (I 8 7 1), who believed that a blind, white isopod from Mammoth Cave,Kentucky, belonged to the marine family Idoteidae. The first portion of Packard's genus is from the Latin caecus,meaning blind. Bowman (1975b) distinguished the first American Asellus of Say (1818) from the Old World genus.This left us with Caecidotea, on the basis of priority, as the genus for many of the asellid species this side of theAtlantic. Unfortunately, many Caecidotea species are pigmented epigean forms with well-developed eyes. Others arewhite and eyeless like the Mammoth Cave species, C. stygia, that Packard described on the basis of a single, damaged,immature specimen!
AMPHIPODA.-The first specimen of what proved to represent a new genus of amphipod crustacean, MexiweckeliaHolsinger and Minckley (1971) was found in Taylor's Spring, shown as a circle in Holsinger and Minckley (1971, Fig.4) and a half-darkened circle in Cole and Minckley (1970, Fig. 40). One morning in August 1968, while I was showingDwight W. Taylor the glass-jar trap used to catch nocturnally emerging Sphaerolana and Speocirolana, he called myattention to a ghostly amphipod trapped on the surface film of the tiny spring pool. The pool turned out to be thetype-locality for Mexiweckelia particeps Holsinger (in Holsinger and Minckley 1971), where it coexisted with thesmaller (ca. 3 mm, rather than 5.5 mm) and more widespread M. colei, known also only from the Cuatro Ciénegasbasin. The serendipitous observation by Taylor led us to a method of collecting these new amphipods, that we believedwere only one species: we vigorously stirred the fibrous plant detritus that lay at the bottom of some small spring-poolsand pits, and searched for animals trapped on the water surface. We returned to Tempe at the end of August with morethan 40 specimens of the tiny eyeless crustaceans. The aid of Dr. John R. Holsinger was sought in further study of theamphipods, for he had published many papers on subterranean peracaridans, and especially on the amphipods.
The amphipods from Taylor's Spring are related to a group of genera that were considered, at the time of their discovery,to be members of the family Gammaridae. That meant that they were the second freshwater gammarids to be reportedfrom México, and they were the second type of subterranean amphipod known from that nation; Bogidiellatabascensis Villalobos-Figueroa 1961 had been described 10 years earlier from a cave in Tabasco. Since then thefamily Bogidiellidae has been redefined (Bousfield 1977) and is no longer considered synonymous with Gammaridae;Holsinger and Longley (1980) assigned Mexiweckelia and related genera to the family Hadziidae. Now the weckeliidgenera are distinguished from other members of the Hadziidae on the basis of differences in uropod 3, the lower lip and,to a lesser extent, the female pereopod 2 (gnathopod 2) (Barnard and Barnard 1983). The Cuatro Ciénegas animalsbelong to the weckeliid group. The freshwater gammarid nearest to México may be some member of the Gammaruspecos complex, perhaps G. hyalelloides Cole 1976.
Meanwhile, in the dozen years that have passed since the description of the two Mexiweckelia species at least ninemore species of subterranean amphipods have been named from México, and three or four undescribed forms have beennoted (Holsinger 1982). Most of these are bogidiellids from southern México (Ruffo and Vigna Taglianti 1977), butMexiweckelia mitchelli was described by Holsinger (1973) from material collected in a Durango cave. Furthermore,two new hadziids (weckeliid group) have been found in the cenotes of Yucatán, Campeche and the Territory of QuintanaRoo, and assigned to a new genus, Mayaweckelia Holsinger (1977). Taxonomic changes have added further to thecomplexity of the Mexican amphipod story.
After the description of the two Mexiweckelia species from Cuatro Ciénegas there were discoveries which suggestedthe genus was rather widespread. Holsinger (1973) described Mexiweckelia texensis from an artesian well in SanMarcos, Texas, in the same paper that reported M. mitchelli. With further study he decided that the Texas speciesrepresented a new genus, Texiweckelia, and the original species of 1973 was found to be comprised of two species ofTexiweckelia, T. texensis and T. insolita, plus a new genus and species Alloweckelia hirsuta. A third species ofTexiweckelia, T. samacos, was also present in the San Marcos well (Holsinger and Longley 1980). The remarkablecongeneric coexistence of three Texiweckelia species lost its meaning when Barnard and Karaman (1982) erected thenew genus Texiweckeliopsis on the basis of T. insolita, and changed T. samacos to Holsingerius samacos, gen.nov. These changes left Mexiweckelia restricted to the Cuatro Ciénegas bolsón and Cueva de la Siquita in Durango.The Durango species still belongs to Mexiweckelia (Holsinger, in litt. July 1983), diminishing the endemism ofCuatro Ciénegas to the level it was when Holsinger and Minckley (1971) reported the discovery of two species of a new,supposedly endemic genus Mexiweckelia. This will be offset when M. particeps becomes Paramexiweckeliaparticeps, a new monotypic genus, known only from Taylor's Spring (Holsinger, in press). We often wondered whichspecies, M. colei or M. particeps, Dr. Taylor spotted on the surface film that day in August 1968; now we can ask,"Which genus did he see?"
All the freshwater hadziids are troglobites or phreatobites; they are found in Eurasia, in North America around theCaribbean, and are associated with the Tethys shores. The bogidiellids are much more widespread, and the discovery ofa new species in the East Indian Archipelago by
Stock (1983) fulfilled his belief in the antiquity of the family, it having reached a nearly world-wide distribution beforethe break up of Pangaea. Bousfield (1982) suggests that the Hadziidae and Bogidiellidae are about the same age andfairly young, going back to the Middle Cretaceous. Whatever the case, at least two bogidiellids have been discovered inSan Marcos, Texas (Holsinger and Longley 1980, Barnard and Barnard 1983) and the gap between Texas and southernMéxico may be closed some day with bogidiellids, as it is today with species of the weckeliid group of the Hadziidae.
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Table 1.-- Crustacea Known from the Bolsón of Cuatro Ciénegas, Coahuila, México.
Branchiopoda
Anostraca
Artemiidae
Artemia Leach 1819
Streptocephalidae
Streptocephalus Baird 1852
Copepoda
Harpactiocoida; Cletodidae
Cletocamptus albuquerquensis (Herrick) 1895
Ostracoda
Podocopa
Cypridae
Chlamydotheca Saussure 1858
Malacostraca
Peracarida
Isopoda
Flabellifera
Cirolanidae
Speocirolana thermydronis Cole and Minckley 1966
Sphaerolana interstitialis Cole and Minckley 1970
Sphaerolana affinis Cole and Minckley 1970
Asellota
Stenasellidae
Mexistenasellus coahuila Cole and Minckley 1972
Amphipoda
Talitroidea
Hyalellidae
Hyalella Smith 1874
Hadzioidea
Hadziidae
Mexiweckelia particeps (Holsinger) in Holsinger and Minckley (1971),
Holsinger in press
Decapoda
Caridea
Palaemonidae
Palaemonetes suttkusi Smalley 1964
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